Ig. Kim et al., CLONING AND EXPRESSION OF HUMAN MITOTIC CENTROMERE-ASSOCIATED KINESINGENE, Biochimica et biophysica acta. Molecular cell research, 1359(3), 1997, pp. 181-186
The human homologue of the hamster mitotic centomere-associated kinesi
n (HsMCAK) gene containing a central type motor domain was isolated fr
om a Jurkat T-cell derived cDNA library. The HsMCAK gene has a predict
ed 723 amino acid open reading frame, encoding a 81 kDa protein that s
hares 79.2% homology with hamster MCAK. Unstimulated T lymphocytes con
tained no detectable HsMCAK-specific mRNA. Activation of resting T-cel
ls by immobilized anti-CD3 resulted in the expression of a 2.9-kb tran
script during the S phase of the cell cycle, The TPA-induced monocytic
differentiation of U937 which also results in growth-arrest abruptly
downregulates the expression of HsMCAK, Removal of TPA restored the gr
owth of the cell through the retrodifferentiation process and the subs
equent expression of HsMCAK. HsMCAK is expressed in tissues containing
dividing cells, such as thymus, testis, small intestine, colon (mucos
al lining), and placenta. These results suggest that the expression of
HsMCAK is first detected in early S phase to support the proliferativ
e response and is strictly regulated at the transcriptional level. (C)
1997 Elsevier Science B.V.