EMBRYONIC PROTEIN-COMPOSITION AND SYNTHESIS DURING IMBIBITION OF WHITE LUPIN SEEDS - EFFECT OF LOW-TEMPERATURES

White lupin seed imbibition under low temperature conditions (8 degree s C) and their subsequent effects on embryonic protein composition and synthesis were investigated. The response to low temperatures was acc ompanied by changes in polypeptide composition and synthesis. The embr yonic axes labelled in vivo with (S-35)-methionine retained their capa city to synthesize proteins during imbibition at 8 degrees C. The synt hesis of some polypeptides was increased during low temperature treatm ent as compared to that at 25 degrees C. These cold-induced polypeptid es were essentially detected in a molecular mass range from 15 to 35 k Da and a pI range from 6.3 to 8.7.