CAPILLARY ISOELECTRIC-FOCUSING

Capillary isoelectric focusing (CIEF) provides excellent resolution of proteins with the advantage that separations are carried out in a cap illary format with on-tube detection and automated analysis. Recent ad vances in the technique provide improved resolution, reproducibility a nd reliability. This review summarizes improvements in sample preparat ion, capillary selection, and focusing and zone mobilization procedure s which have helped CIEF become a more robust analytical method. New a pplications are reviewed, in particular the use of CIEF in analysis of biopharmaceutical products.