AFFINITY CAPILLARY ELECTROPHORESIS

Authors
RIPPEL G CORSTJENS H BILLIET HAH FRANK J
Citation
G. Rippel et al., AFFINITY CAPILLARY ELECTROPHORESIS, Electrophoresis, 18(12-13), 1997, pp. 2175-2183
Citations number
81
Categorie Soggetti
Biochemical Research Methods","Chemistry Analytical
Journal title
Electrophoresis → ACNP
ISSN journal
01730835
Volume
18
Issue
12-13
Year of publication
1997
Pages
2175 - 2183
Database
ISI
SICI code
0173-0835(1997)18:12-13<2175:ACE>2.0.ZU;2-F
Abstract
The application of affinity capillary electrophoresis (ACE) to the stu dy of molecular interactions is reviewed. ACE appears to be a sensitiv e, versatile and convenient tool to obtain reliable data on binding co nstants and stoichiometries of interacting systems using the Hummel-Dr eyer method and variants thereof. A powerful feature is the possibilit y to analyze simultaneously the affinity of a large number of compound s for the same ligand, making it a promising tool for the screening of large combinatorial libraries.