PRE-COLUMN, ON-COLUMN AND POSTCOLUMN DERIVATIZATION IN CAPILLARY ELECTROPHORESIS

This survey gives a short overview of the various reagents and procedu res that can be used for pre-, post-and on-column derivatization in ca pillary electrophoresis. First there is an introduction about capillar y electrophoresis as an analytical technique; this is followed by a di scussion of the pros and cons of the various modes of derivatization a nd a comparison with liquid chromatography. In the following paragraph s the reagents for a number of functional groups are discussed. The em phasis is on derivatization of the amino group. Most of the informatio n on the reagents and derivatization procedures is listed in tables to gether with information on the detection mode, analytes, sensitivity a nd samples. In addition to the amino group, information is given on la belling of aldehyde, keto, carboxyl, hydroxyl and sulfhydryl groups.