CAPILLARY ELECTROPHORETIC SEPARATIONS OF ENANTIOMERS USING CYCLODEXTRIN-CONTAINING BACKGROUND ELECTROLYTES

The 1996 primary literature papers which deal with the separation of e nantiomers using cyclodextrins are reviewed here. Though the majority of the papers still use the neutral native cyclodextrins or the neutra l derivatised cyclodextrins as resolving agents, there was a significa nt increase in number of separations which relied on charged cyclodext rins, both weak electrolytes and strong electrolytes, as resolving age nts. Also, there was an increase in the number of papers which reporte d binding constants and correlated them with other physical or chemica l characteristics of the analytes. Several successful minor enantiomer determinations were presented, pushing the reliable quantitation leve ls below 0.1%. Work continued on the simultaneous use of neutral and c harged cyclodextrins to improve separation selectivity or peak resolut ion.