CAPILLARY-ZONE-ELECTROPHORESIS OF PROTEINS

This review article with 237 references is focused on capillary zone e lectrophoresis (CZE) of proteins. It includes discussion of modelling electrophoretic migration of proteins, sample pretreatment before the analysis, methods reducing the sorptions of proteins on the capillary wall, and techniques for increasing selectivity by using electrolyte a dditives including the sieving matrices. Significant progress in detec tion techniques, namely in laser-induced fluorescence and mass spectro metry, is emphasized. Modifications of CZE using specific interactions , such as affinity capillary electrophoresis or capillary immunoelectr ophoresis, are debated as well as combination of CZE with other separa tion methods such as high performance liquid chromatography (HPLC). A number of practical applications of CZE of proteins are described.