A NOVEL RESTRICTION-ENDONUCLEASE UNBI, A NEOSCHIZOMER OF SAU96I FROM AN UNIDENTIFIED PSYCHROFILIC BACTERIUM FROM ANTARCTICA IS INHIBITED BYPHOSPHATE IONS

Authors
KAWALEC M BORSUK P PIECHULA S STEPIEN PP
Citation
M. Kawalec et al., A NOVEL RESTRICTION-ENDONUCLEASE UNBI, A NEOSCHIZOMER OF SAU96I FROM AN UNIDENTIFIED PSYCHROFILIC BACTERIUM FROM ANTARCTICA IS INHIBITED BYPHOSPHATE IONS, Acta Biochimica Polonica, 44(4), 1997, pp. 849-852
Citations number
3
Categorie Soggetti
Biology
Journal title
Acta Biochimica Polonica → ACNP
ISSN journal
0001527X
Volume
44
Issue
4
Year of publication
1997
Pages
849 - 852
Database
ISI
SICI code
0001-527X(1997)44:4<849:ANRUAN>2.0.ZU;2-G
Abstract
A novel type II restriction endonuclease UnbI was isolated from an uni dentified psychrofilic bacterial strain from Antarctica. UnbI recogniz es and cleaves the sequence 5'-GGNCC-3', producing 5 nucleotide long s ticky ends. In this respect it differs from its neoschizomer Sau96I an d all other restriction enzymes recognizing this sequence. UnbI has a relatively low temperature optimum of 15 degrees C to 20 degrees C and its activity is completely inhibited by inorganic phosphate.