EFFECT OF CEREBRAL-ISCHEMIA ON DOPAMINE-RECEPTORS AND UPTAKE SITES INTHE GERBIL HIPPOCAMPUS

Dopamine D-1 and D-2 receptors and uptake sites were studied in the ge rbil hippocampus, parietal cortex and thalamus 1 h to 7 days after 10 min of cerebral ischemia using the occlusion of bilateral common carot id arteries. [H-3]SCH23390 4,5-tetrahydro-3-methyl-5-phenyl-7-ol-benza zepine) and [H-3]mazindol were used as markers of dopamine D-1 recepto rs and uptake sites, respectively. [H-3]Nemonapride was used to label dopamine D-2 receptors. No obvious alteration in [H-3]SCH23390 and [H- 3]mazindol binding was found in the hippocampus up to 48 h after ische mia. These bindings showed a significant reduction in the hippocampus after 7 days of recirculation. In contrast, [H-3]nemonapride binding w as unaffected in the hippocampus during the recirculation periods. The parietal cortex and thalamus also exhibited no significant changes in [H-3]SCH23390, [H-3]nemonapride and [H-3]mazindol binding after ische mia. MAP2 (microtubule-associated protein 2) immunoreactivity was unch anged in all regions up to 48 h after ischemia. Thereafter, a marked l oss of MAP2-immunoreactive neurons was observed in the hippocampal CA1 and CA3 neurons 7 days after recirculation. These findings were consi stent with histological observations with cresyl violet staining. Our results demonstrate that dopamine D-1 receptors and dopamine uptake si tes in the hippocampus are susceptible to cerebral ischemia, whereas d opamine D-2 receptors in this region are particularly resistant. Furth ermore, these findings suggest that dopamine transmission may not be m ajor factor in producing ischemic hippocampal damage. (C) 1997 Elsevie r Science B.V.