IRS-PCR-BASED GENETIC-MAPPING OF THE HUNTINGTIN INTERACTING PROTEIN GENE (HIP1) ON MOUSE-CHROMOSOME-5

Huntington's disease (HD) is a devastating central nervous system diso rder. Even though the gene responsible has been positionally cloned re cently, its etiology has remained largely unclear. To investigate pote ntial disease mechanisms, we conducted a search for binding partners o f the HD-protein huntingtin. With the yeast two-hybrid system, one suc h interacting factor, the huntingtin interacting protein-1 (HIP-1), wa s identified (Wanker et al. 1997; Kalchman et al. 1997) and the human gene mapped to 7q11.2. In this paper we demonstrate the localization o f the HIP1 mouse homologue (Hip1) into a previously identified region of human-mouse synteny on distal mouse Chromosome (Chr) 5, both employ ing an IRS-PCR-based mapping strategy and traditional fluorescent in s itu hybridization (FISH) mapping.