SPECTROPHOTOMETRY OF HEMOGLOBIN - ABSORPTION-SPECTRA OF BOVINE OXYHEMOGLOBIN, DEOXYHEMOGLOBIN, CARBOXYHEMOGLOBIN, AND METHEMOGLOBIN

The absorptivity at 540 nm of bovine hemiglobincyanide (cyanmethemoglo bin) was determined on the basis of the iron content and found to be e qual to the established value for human hemiglobincyanide (11.0 L . mm ol(-1).cm(-1)). On this basis the absorption spectra of the common der ivatives were determined for bovine hemoglobin. There proved to be onl y slight differences in the oxyhemoglobin, deoxyhemoglobin, and carbox yhemoglobin spectra between bovine and human hemoglobin. For compariso n of the methemoglobin spectra a new series of measurements was made f or human hemoglobin. As also found in the rat, the methemoglobin spect rum of bovine blood differed considerably from that in the human. Thes e differences should be taken into account in multicomponent analysis. (C) 1991 Elsevier Science Inc.