Wg. Zijlstra et A. Buursma, SPECTROPHOTOMETRY OF HEMOGLOBIN - ABSORPTION-SPECTRA OF BOVINE OXYHEMOGLOBIN, DEOXYHEMOGLOBIN, CARBOXYHEMOGLOBIN, AND METHEMOGLOBIN, Comparative biochemistry and physiology. B. Comparative biochemistry, 118(4), 1997, pp. 743-749
The absorptivity at 540 nm of bovine hemiglobincyanide (cyanmethemoglo
bin) was determined on the basis of the iron content and found to be e
qual to the established value for human hemiglobincyanide (11.0 L . mm
ol(-1).cm(-1)). On this basis the absorption spectra of the common der
ivatives were determined for bovine hemoglobin. There proved to be onl
y slight differences in the oxyhemoglobin, deoxyhemoglobin, and carbox
yhemoglobin spectra between bovine and human hemoglobin. For compariso
n of the methemoglobin spectra a new series of measurements was made f
or human hemoglobin. As also found in the rat, the methemoglobin spect
rum of bovine blood differed considerably from that in the human. Thes
e differences should be taken into account in multicomponent analysis.
(C) 1991 Elsevier Science Inc.