MULTIDOMAIN STRUCTURE OF THE RAPANA-THOMASIANA (GASTROPOD) HEMOCYANINSTRUCTURAL SUBUNIT RHSS1

Authors
STOEVA S DOLASHKA P PERVANOVA K GENOV N VOELTER W
Citation
S. Stoeva et al., MULTIDOMAIN STRUCTURE OF THE RAPANA-THOMASIANA (GASTROPOD) HEMOCYANINSTRUCTURAL SUBUNIT RHSS1, Comparative biochemistry and physiology. B. Comparative biochemistry, 118(4), 1997, pp. 927-934
Citations number
41
Categorie Soggetti
Biology,Zoology
Journal title
Comparative biochemistry and physiology. B. Comparative biochemistry → ACNP
ISSN journal
03050491
Volume
118
Issue
4
Year of publication
1997
Pages
927 - 934
Database
ISI
SICI code
0305-0491(1997)118:4<927:MSOTR(>2.0.ZU;2-6
Abstract
The Rapana thomasiana hemocyanin structural subunit RHSS1 is composed of eight functional dioxygen-binding domains. To determine the multido main structure, the polypeptide chain of RHSS1 was subjected to limite d proteolysis with TPCK-trypsin, elastase and other proteinases. Indiv idual functional units and fragments, containing two or three domains, were isolated and characterized. All domains and fragments were N-ter minally sequenced and the order of the dioxygen binding units in the p olypeptide chain of RHSS1 was established. (C) 1997 Elsevier Science I nc.