IMPROVED TRANSLATIONAL EFFICIENCY OF SUBTILISIN YAB GENE WITH DIFFERENT INITIATION CODONS IN BACILLUS-SUBTILIS AND ALKALOPHILIC BACILLUS YAB

Authors
YEH CM CHANG HK HSIEH HM YODA K YAMASAKI M TSAI YC
Citation
Cm. Yeh et al., IMPROVED TRANSLATIONAL EFFICIENCY OF SUBTILISIN YAB GENE WITH DIFFERENT INITIATION CODONS IN BACILLUS-SUBTILIS AND ALKALOPHILIC BACILLUS YAB, Journal of applied microbiology, 83(6), 1997, pp. 758-763
Citations number
25
Journal title
Journal of applied microbiology → ACNP
ISSN journal
13645072
Volume
83
Issue
6
Year of publication
1997
Pages
758 - 763
Database
ISI
SICI code
1364-5072(1997)83:6<758:ITEOSY>2.0.ZU;2-1
Abstract
The ale gene specifying the subtilisin YaB produced by alkalophilic Ba cillus YaB, has an unusual start codon UUG. Changing this codon to AUG and GUG increased expression of the ale gene in B. subtilis DB104 and in an ale deficient mutant strain YaB-DEC4. The relative translationa l efficiency order of the three initiation codons is AUG; > GUG > UUG in B. subtilis DB104 and in YaB-DEC4. These data suggest that the pref erred initiation codon is AUG for ale gene expression in Bacillus.