D. Lamosova et al., INFLUENCE OF MELATONIN ON CHICK SKELETAL-MUSCLE CELL-GROWTH, Comparative biochemistry and physiology. C. Comparative pharmacologyand toxicology, 118(3), 1997, pp. 375-379
Melatonin as a highly lipophilic compound readily enters all subcellul
ar compartments and acts by various mechanisms. Tn the present study,
we investigated the effects of different concentrations of melatonin i
n medium (physiological and supraphysiological doses) at two treatment
times (48 and 120 hr) on growth and changes of growth parameters of c
ultured chick embryonic skeletal muscle cells. The physiological doses
of melatonin (100 pg to 10 ng/ml of medium) stimulated proliferation
of cells and raised DNA, RNA protein contents and an incorporation of
[H-3]leucine into cell protein after 48 hr of treatment. The prolongat
ion of exposure to melatonin in the physiological dose to 120 hr or an
increase of melatonin dose to a supraphysiological one evoked the inh
ibition of cell growth and proliferation by decreasing the number of c
ells and the amount of labeled leucine incorporated into cell protein.
Results demonstrate that melatonin's action in these cells is time an
d dose dependent. (C) 1997 Elsevier Science Inc.