T. Chiba et R. Ohi, DO WE STILL NEED TO COLLECT STOOL - EVALUATION OF VISUALIZED FATTY-ACID ABSORPTION - EXPERIMENTAL STUDIES USING RATS, JPEN. Journal of parenteral and enteral nutrition, 22(1), 1998, pp. 22-26
Background: Short-gut syndrome is likely to impair enteric fat utiliza
tion. This study was undertaken to develop a clinical test of lipid ab
sorption without fecal collection. Methods: The absorption of enterall
y fed radioactive long-chain fatty acid, beta-methyl-p-(I-123)-iodophe
nylpentadecanoic acid was investigated with continuous chyle collectio
n in rats. The changes in excretion and time-dependent biodistribution
of radioactivity of the enterally fed agent were assessed in normal c
ontrol animals. Similarly, sequential urinary excretion and biodistrib
ution were studied along with scintigraphy using sham-operated and sho
rt-gut animals. Results: Approximately 64% of the enterally fed radioa
ctivity was recovered in the collected chyle (24 hours). A comparison
of normal control, sham-operated, and short-gut animals showed signifi
cantly less urinary and greater fecal excretions of radioactivity in s
hort-gut animals. With the use of sequential scintigraphy, the small i
ntestine, whole-body soft tissues, and urinary bladder were well visua
lized in sham-operated animals, whereas the large intestine and feces
were demonstrated earlier in short-gut animals. Conclusions: Our resul
ts suggest that enteral feeding of the agent might be feasible for det
ermining lipid absorption from the the dynamic changes of radioactivit
y in visualized abdominal organs and in urine.