Y. Edashige et T. Ishii, RHAMNOGALACTURONAN-I FROM XYLEM DIFFERENTIATING ZONES OF CRYPTOMERIA-JAPONICA, Carbohydrate research, 304(3-4), 1997, pp. 357-365
Rhamnogalacturonan I (RG-I) was isolated from the pectin of the cell w
all of xylem differentiating zones of sugi (Cryptomeria japonica D. Do
n, gymnosperm) by digesting with endo-alpha-1,4-polygalacturonase foll
owed by gel-permeation chromatography. Structure of RG-I was character
ized by glycosyl composition and glycosyl linkage analyses, partial ac
id hydrolysis, and lithium (Li)-treatment in ethylenediamine. The olig
osaccharides produced by partial acid hydrolysis showed the backbone o
f RG-I to be a disaccharide repeating unit of --> 4)-alpha-D-GalpA-(1
--> ,2)-alpha-L-Rhap-(1 -->. Arabinan and oligogalactosyl side chains
were attached at O-4 of rhamnosyl residues of the backbone. Glycosyl c
omposition and glycosyl linkage analyses suggested that the structures
and/or the distribution of the arabinan sidechains of sugi RG-I were
different from those of sycamore (angiosperm) RG-I. (C) 1997 Elsevier
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