OPTIMIZATION OF POLYMERASE CHAIN-REACTION (PCR) FOR DETECTION AND CHARACTERIZATION OF SHIGATOXIN PRODUCING ESCHERICHIA-COLI (STEC) IN FOOD

Authors
GALLIEN P RICHTER H MUCH C FROBE I PERLBERG KW PROTZ D
Citation
P. Gallien et al., OPTIMIZATION OF POLYMERASE CHAIN-REACTION (PCR) FOR DETECTION AND CHARACTERIZATION OF SHIGATOXIN PRODUCING ESCHERICHIA-COLI (STEC) IN FOOD, Berliner und Munchener Tierarztliche Wochenschrift, 110(11-12), 1997, pp. 422-426
Citations number
29
Categorie Soggetti
Veterinary Sciences
Journal title
Berliner und Munchener Tierarztliche Wochenschrift → ACNP
ISSN journal
00059366
Volume
110
Issue
11-12
Year of publication
1997
Pages
422 - 426
Database
ISI
SICI code
0005-9366(1997)110:11-12<422:OOPC(F>2.0.ZU;2-8
Abstract
The polymerase chain reaction (PCR) was improved to detect shigatoxin producing Escherichia coli (STEC) in milk. Numbers of colony forming u nits (cfu) in test samples, concentrations and types of primers, amoun t of MgCl2, types of thermostable DNA-Polymerase, and cycling programs were modified up to obtain the cleanest electrophoretic pattern and t he highest sensitivity. Experimental conditions for further characteri zation of STEC-isolates by means of PCR are given by summarizing data from literature.