INVESTIGATION OF THE EFFECT OF LANNATE ON KINETIC-PARAMETERS OF ACETYLCHOLINESTERASE - SLIGHTLY CONCAVE MIXED-TYPE OF INHIBITION SYSTEM

The effect of lannate on kinetic parameters of camel retina acetylchol inesterase is investigated in the present study. The K-s for the hydro lysis of acetylthiocholine iodide was found to be 0.086 mM in the cont rol system; a value that increased in the lannate treated systems. The V-max was 0.853 mu mole/min/mg protein for the control system while i t decreased in the lannate treated systems. Dixon as well as Lineweave r-Burk plots and their secondary replots indicated that the nature of the inhibition is of the slightly concave mixed type, which is conside red to be a 'semi-pure competitive' and 'semi-partial as well as semi- pure non-competitive' mixture. The values of K-i(slope) and K-I(interc ept) were estimated as 0.143 mu M and 0.179 mu M respectively. The K-I was greater than K-i indicating that lannate has a greater affinity o f binding for the peripheral site than the active site of the camel re tina AChE.