The present work characterizes parameters of ubiquitin turnover in Chl
amydomonas reinhardtii Dangeard growing under constant temperature con
ditions and after an exposure to cold shock. The ratio of free and con
jugated ubiquitin to total protein and the rate constant of ubiquitin
synthesis and conjugation increased about 2-fold during the first 4 h
after cold treatment, whereas the rate constant of ubiquitin degradati
on reached its maximum 8 h after treatment. The half-life of ubiquitin
calculated from the constant of degradation decreased from 6 h to 3.5
h during the first 4 h after completion of the cold treatment. The ra
te constant of ubiquitin deconjugation did not change after cold treat
ment. The ratio of free to conjugated ubiquitin decreased temporarily
to approximately 8 immediately after cold treatment and increased back
to its original value of about 11 at 2 h after cold treatment. These
observations raise questions regarding the regulatory mechanisms of ub
iquitin synthesis and degradation.