A FIRST-GENERATION WHOLE GENOME RADIATION HYBRID MAP SPANNING THE MOUSE GENOME

Authors
MCCARTHY LC TERRETT J DAVIS ME KNIGHTS CJ SMITH AL CRITCHER R SCHMITT K HUDSON J SPURR NK GOODFELLOW PN
Citation
Lc. Mccarthy et al., A FIRST-GENERATION WHOLE GENOME RADIATION HYBRID MAP SPANNING THE MOUSE GENOME, PCR methods and applications, 7(12), 1997, pp. 1153-1161
Citations number
23
Journal title
PCR methods and applications → ACNP
ISSN journal
10549803
Volume
7
Issue
12
Year of publication
1997
Pages
1153 - 1161
Database
ISI
SICI code
1054-9803(1997)7:12<1153:AFWGRH>2.0.ZU;2-2
Abstract
We have assembled a first-generation anchor map of the mouse genome us ing a panel of 94 whole-genome-radiation hybrids (WG-RHs) and 271 sequ ence-tagged sites (STSs). This is the first genome-wide RH anchor map of a model organism. All of the STSs have been previously localized on the genetic map and are located 8.8 Mb apart on average. This mouse W G-RH panel, known as T31, has an average retention frequency of 27.6% and an estimated potential resolution of 145 kb, making it a powerful resource for efficient large-scale expressed sequence tag mapping.