PRODUCTION OF RESPIRABLE VESICLES CONTAINING LIVE LEGIONALLA-PNEUMOPHILA CELLS BY 2 ACANTHAMOEBA SPP

Authors
BERK SG TING RS TURNER GW ASHBURN RJ
Citation
Sg. Berk et al., PRODUCTION OF RESPIRABLE VESICLES CONTAINING LIVE LEGIONALLA-PNEUMOPHILA CELLS BY 2 ACANTHAMOEBA SPP, Applied and environmental microbiology, 64(1), 1998, pp. 279-286
Citations number
17
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Applied and environmental microbiology → ACNP
ISSN journal
00992240
Volume
64
Issue
1
Year of publication
1998
Pages
279 - 286
Database
ISI
SICI code
0099-2240(1998)64:1<279:PORVCL>2.0.ZU;2-8
Abstract
Two Acanthamoeba species, fed at three temperatures, expelled vesicles containing living Legionella pneumophila cells. Vesicles ranged from 2.1 to 6.4 mu m in diameter and theoretically could contain several hu ndred bacteria. Viable L. pneumophila cells were observed within vesic les which had been exposed to two cooling tower biocides for 24 h. Clu sters of bacteria in vesicles were not dispersed by freeze-thawing and sonication. Such vesicles may be agents for the transmission of legio nellosis associated with cooling towers, and the risk may be underesti mated by plate count methods.