DIFFERENTIATION OF CAMPYLOBACTER ISOLATES ON THE BASIS OF SENSITIVITYTO BOILING IN WATER AS MEASURED BY PCR-DETECTABLE DNA

Authors
MOHRAN ZS ARTHUR RR OYOFO BA PERUSKI LF WASFY MO ISMAIL TF MURPHY JR
Citation
Zs. Mohran et al., DIFFERENTIATION OF CAMPYLOBACTER ISOLATES ON THE BASIS OF SENSITIVITYTO BOILING IN WATER AS MEASURED BY PCR-DETECTABLE DNA, Applied and environmental microbiology, 64(1), 1998, pp. 363-365
Citations number
12
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Applied and environmental microbiology → ACNP
ISSN journal
00992240
Volume
64
Issue
1
Year of publication
1998
Pages
363 - 365
Database
ISI
SICI code
0099-2240(1998)64:1<363:DOCIOT>2.0.ZU;2-9
Abstract
Differential sensitivity for the release of PCR-detectable genomic DNA upon boiling in water is reported for 45 Campylobacter jejuni and Cam pylobacter coli strains isolated in Egypt. All of the strains released PCR-detectable DNA when treated with proteinase K and sodium dodecyl sulfate. When DNA was extracted from these strains by boiling in water , nine (20%) of the strains were PCR negative or resistant to boiling, suggesting the presence of boiling-sensitive and boiling-resistant ph enotypes.