The concentration of fluoride was determined in water, forage and urin
e and serum samples of buffaloes from the Unnao district of India. The
water and forage samples contained 2.01 +/- 0.51 and 22.50 +/- 0.82 p
pm of fluoride, respectively. The analysis of biosamples collected fro
m the affected animals revealed higher levels of fluoride in serum (0.
58 +/- 0.05 ppm) and urine (10.64 +/- 1.23 ppm). Clinical examination
identified a 40.34% prevalence rate of clinical lesions suggestive of
fluorosis in buffalo of this locality. Dental lesions were present inv
ariably in all affected animals whereas lameness, painful bony exostos
is and emaciation were recorded in 28.17%, 8.45% and 76.00% of the ani
mals. Based on the clinical lesions and fluoride content in water, ser
um and urine, it was concluded that the problem of fluorosis in buffal
o is attributable to drinking water containing toxic levels of fluorid
e. (C) 1997 Elsevier Science B.V.