A LINKAGE MAP OF THE PEA (PISUM-SATIVUM L) GENOME CONTAINING CLONED SEQUENCES OF KNOWN FUNCTION AND EXPRESSED SEQUENCE TAGS (ESTS)

A linkage map of the pea (Pisum sativum L.) genome is presented which is based on F-2 plants produced by crossing the marrowfat cultivar 'Pr imo' and the blue-pea breeding line 'OSU442-15'. This linkage map cons ists of 209 markers and covers 1330 cM (Kosambi units) and includes RF LP, RAPD and AFLP markers. By mapping a number of anchor loci, the 'Pr imo' x 'OSU442-15' map has been related to other pea linkage maps. A f eature of the map is the incorporation of 29 loci representing genes o f known function, obtained from other laboratories. The map also conta ins RFLP loci detected using sequence-characterized cDNA clones develo ped in our laboratory. The putative identities of 38 of these cDNA clo nes were assigned by examining public-sequence databases for protein o r nucleotide-sequence similarities. The conversion of sequence-charact erized pea cDNAs into PCR-amplifiable and polymorphic sequence-tagged sites (STSs) was investigated using 18 pairs of primers designed for s ingle-copy sequences. Eleven polymorphic STSs were developed.