MIGRATION AND BIDIRECTIONAL PHOTOTAXIS IN DICTYOSTELIUM-DISCOIDEUM SLUGS LACKING THE ACTIN CROSS-LINKING 120 KDA GELATION FACTOR

Three mutant strains of Dictyostelium discoideum, lacking different ac tin-binding proteins, were tested for behavioural deficits in the mult icellular pseudoplasmodium (slug) stage, Two strains, defective in the production of either alpha-actinin (an actin cross-linker) or severin (an actin capping and severing protein), did not show changes in slug behaviour, Slugs of the mutant lacking another actin cross-linker, th e 120 kDa gelation factor (ABP-120), however, migrated shorter distanc es in darkness as well as in horizontally directed light. More remarka bly, they migrated at an angle of approximately 45 degrees to the left or right of the incident light, whereas wild-type slugs migrated on f airly straight paths towards the light. We discuss the hypothesis that this bidirectional oblique-angle phototaxis is due to changes in the optical properties of the pseudoplasmodia. Normally, in wild-type slug s, a lens effect causes stronger stimulation on the side distal to the incident light. We propose that in the mutant the lens quality is red uced, so that at small angles between the slug axis and the rays of li ght the proximal side is stimulated more intensely. As a result, the i ntended symmetrical stimulation is achieved at a certain angle to the left or right of the incident light. We assume that the absence of ABP -120 alters the shape of the lens and/or enhances internal light scatt ering via degradation of intercellular coherence; however, intracellul ar attenuation of light remains an additional or alternative possibili ty.