OXIDATION INTERMEDIATES OF ALPHA-GLUCOSYL RUTIN BY PULSE-RADIOLYSIS

Transient intermediates generated in the reaction of alpha-glucosyl ru tin (G-rutin) with the OH radical or the azide radical were investigat ed by pulse radiolysis. The OH radical reacted with G-rutin to produce the OH-adduct radical, followed by deprotonation in neutral and acidi c solutions. In alkaline solutions, the OH-adduct di-anion radical for med from the di-anions of G-rutin were dehydrated to the phenoxyl radi cal. On the other hand, G-rutin was oxidized to one-electron oxidized cation radical by the azide radical. The pK(a) values of the OH radica l adduct were found to be 6.83 +/- 0.10 and 8.87 +/- 0.13. While, the pK(a) values of the phenoxyl radical of G-rutin were found to be 7.52 +/- 0.11 and 9.95 +/- 0.13. (C) 1997 Published by Elsevier Science Ltd . All rights reserved.