A NOVEL PUTATIVE G-PROTEIN-COUPLED RECEPTOR EXPRESSED IN LUNG, HEART AND LYMPHOID-TISSUE

cDNA encoding a novel putative G-protein-coupled receptor, named LyGPR (lymphocyte derived G-protein-coupled receptor) was cloned using a re verse transcription-PCR approach, The LyGPR amino acid sequence is 375 residues long and shows similarity (about 30-35% identity) both to th e angiotensin receptors and members of the chemokine receptor family, Northern blot analysis revealed a 3.1-kb LyGPR transcript expressed pr edominantly in lung, heart and lymphoid tissues, LyGPR expression was also detected in the pre-B acute lymphoblastoid leukemia cell lines Re h and Nalm-6, in the Burkitt's lymphoma line Daudi, and in hematopoiet ic progenitor cells from bone marrow, as well as in B cells, T cells a nd monocytes from peripheral blood. (C) 1997 Federation of European Bi ochemical Societies.