ANTIMICROBIAL FUNCTIONS OF HUMAN MONOCYTES DEPEND ON CONCENTRATION OFGLUTAMINE IN-VITRO

We investigated the impact of different concentrations of glycyl gluta mine on antimicrobial functions of human monocytes in vitro. Generatio n of suproxide anion, phagocytosis and killing capacity were measured as an expression of antimicrobial activity after an incubation period of 38 h at glycyl glutamine concentrations of 0.0, 0.4, and 2.0 mmol/l respectively. For each of the tests, significantly higher results wer e obtained with concentrations of glycyl glutamine of 0.4 mmol/l as co mpared to 0.0 mmol/l (p < 0.01). Further increases of superoxide anion production (p < 0.05) and killing capacity (p = 0.053) were observed when glycyl glutamine concentration in the culture medium was raised t o 2.0 mmol/l. Variation of glycyl glutamine concentrations in vitro si gnificantly affects antimicrobial functions of human monocytes.