We have used energy-filtered electron microscopy and electron energy l
oss spectroscopy (EELS) to characterize the distributions of sulfur-ri
ch proteins in granular layer cells of squamous stratifying epithelia
and their redistribution in the cornified layer, with particular atten
tion to assembly of the cornified cell envelope (CE). Our measurements
provide quantitative information that complements highly specific but
qualitative data from immunocytochemistry. Spatial distributions of s
ulfur, phosphorus, and nitrogen were mapped in unstained thin sections
of mouse epidermis and forestomach, using a postcolumn energy filter.
Nitrogen images were indicative of total protein while phosphorus ima
ges provided a control to validate the algorithms used to calculate th
e elemental maps. Sulfur was found at high levels in round L-granules
in the granulocyte cytoplasm and in the cornified CE, correlating with
the presence of the protein, loricrin (similar to 7% Cys/Met residues
). EELS confirmed these observations quantitatively: either L-granules
consist exclusively of loricrin or any additional components must hav
e an equally high net sulfur content. These data also confirm the larg
e fraction (similar to 75%) of loricrin in the CE, as inferred from mo
deling of its amino acid composition. We also observed extracellular d
eposits between cornified squames in fetal mouse epidermis that we cal
l peripheral granules. Their sulfur content is at least as high as tha
t of L-granules but they do not label with anti-loricrin antibodies, s
uggesting the presence of another sulfur-rich protein.