V. Viyanant et al., DIAGNOSIS OF CATTLE FASCIOLIASIS BY THE DETECTION OF A CIRCULATING ANTIGEN USING A MONOCLONAL-ANTIBODY, Asian Pacific Journal of Allergy and Immunology, 15(3), 1997, pp. 153-159
A monoclonal antibody (MoAb) 1C12 that reacts with a 66 kDa surface te
gumental (ST) antigen of adult worms of Fasciola gigantica was used to
detect circulating antigen in sera of experimentally and naturally in
fected cattle. A combination of rabbit anti ST-antigens and MoAb 1C12
were used to capture and detect the circulating antigen in sandwich EL
ISA, The dilutions of 1:1,000 of rabbit anti ST-antigens and 1:100 for
MoAb 1C12 were used to reduce cross-reactivity with other trematodes'
antigens. The circulating antigen of F. gigantica was demonstrated in
sera of all experimentally infected animals as early as the first wee
k after the infection, and it remained detectable until the experiment
was terminated at week 32 after the infection. Of the 97 serum sample
s from naturally infected cattle, the sensitivity of 86.6% was observe
d when the cut-off point was calculated from 32 serum specimens from u
ninfected control calves. The sensitivity increased to 100% when the c
ommercial fetal calf and trematode-free baby calves sera were used for
calculation of the control cut-off point. Based on these results, the
combination of rabbit anti ST-antigens and MoAb 1C12 sandwich ELISA a
ppeared to be sensitive, specific, and applicable in the immunodiagnos
is of fasciolosis in cattle for epidemiological study and monitoring o
f chemotherapeutic efficacy.