ESTABLISHMENT OF A NEW MURINE-PHENOTYPIC ANGIOSARCOMA CELL-LINE (ISOS-1)

A cell line, designated ISOS-1, was established from a tumor formed by transplantation of a human angiosarcoma into mice with severe combine d immunodeficiency (SCID). The cells showed endothelial properties, ba sed on the uptake of Dil-Ac-LDL and binding of UEA-I/GSA-I lectins, bu t were negative for CD11b and Pan Cytokeratin. However, the cells lost differentiated characteristics such as expression of von Willebrand f actor, contact inhibition growth and tube formation activity. These fi ndings indicate that ISOS-1 is a poorly-differentiated endothelial cel l line. At the 81st, passage, all of the cells were positive for H-2D( d) in various intensity, but not HLA-ABC. The metaphase chromosomes co nsistently showed a characteristic mouse, but not human, telocentric f orm. Furthermore, this cell line produced fatal tumor growth in SCID m ice and also in BALB/c mice. These results suggest that ISOS-1 is a mu rine-phenotypic angiosarcoma cell line. (C) 1998 Elsevier Science Irel and Ltd.