A 3-STEP PCR PROTOCOL FOR CONSTRUCTION OF CHIMERIC PROTEINS

Authors
GRANDORI R STRUCK K GIOVANIELLI K CAREY J
Citation
R. Grandori et al., A 3-STEP PCR PROTOCOL FOR CONSTRUCTION OF CHIMERIC PROTEINS, Protein engineering, 10(9), 1997, pp. 1099-1100
Citations number
11
Categorie Soggetti
Biothechnology & Applied Migrobiology",Biology
Journal title
Protein engineering → ACNP
ISSN journal
02692139
Volume
10
Issue
9
Year of publication
1997
Pages
1099 - 1100
Database
ISI
SICI code
0269-2139(1997)10:9<1099:A3PPFC>2.0.ZU;2-C
Abstract
A general method is described for creating chimeric proteins by transp osition of subdomain-sized gene fragments. The method uses three seque ntial PCR steps to circumvent several limitations inherent in transpos ition of small insertions and has been optimized to avoid purification of intermediate products. The sequence-independence of the method per mits flexibility in the choice of host molecule and insertion site.