The same quantity of a fluorescein labelled dextran was injected into
the vitreous humor of 5 rabbits; in one eye, disserved in 10 mu l solu
tion and in the other, 100 mu l. The level of fluorescence in the two
eyes was compared a few days later and found to average only 12% less
with the larger volume. It is concluded that regurgitation through the
needle hole can be ignored in spite of the very high intraocular pres
sure created by the 100 mu l injection.