COINCIDENT INDUCTION OF K-REV-1 RAP-1A, RAP-1B AND H-RAS MESSENGER-RNAS IN THE RAT SPINAL-CORD BY NOXIOUS-STIMULATION

Two cDNA fragments, Krev-1/rap 1A and rap 1B, were amplified from tota l cellular RNA of the rat spinal cord by reverse transcription-polymer ase chain reaction with a set of oligonucleotide primers specific for the human rap 1A cDNA. We report here using Northern blot analysis wit h these cDNA probes that noxious stimulation causes a marked and coinc ident increase in rap 1A, rap 1B and H-ras mRNAs in the rat spinal cor d. This suggests that Rap 1 participates in sensory processing in spin al neurons in parallel with Ras. (C) 1997 Elsevier Science B.V.