A SHORTER ENRICHMENT CULTURE FOR THE ISOLATION OF ESCHERICHIA-COLI O157-H7 FROM RAW MINCED BEEF

Authors
VERNOZYROZAND C MAZUY C RAYGUENIOT S BOUTRANDLOEI S MEYRAND A RICHARD Y
Citation
C. Vernozyrozand et al., A SHORTER ENRICHMENT CULTURE FOR THE ISOLATION OF ESCHERICHIA-COLI O157-H7 FROM RAW MINCED BEEF, Revue de Medecine Veterinaire, 148(11), 1997, pp. 879-882
Citations number
15
Categorie Soggetti
Veterinary Sciences
Journal title
Revue de Medecine Veterinaire → ACNP
ISSN journal
00351555
Volume
148
Issue
11
Year of publication
1997
Pages
879 - 882
Database
ISI
SICI code
0035-1555(1997)148:11<879:ASECFT>2.0.ZU;2-A
Abstract
The use of immunomagnetic separation (IMS) techniques has been reporte d to reduce the total test time and improve the sensitivity of microbi ological tests done on foods. This study demonstrates the ability of t he Dynabeads(TM) immunomagnetic separation system, to enable detection of a few cells of E. coli O157:H7 in 25g of inoculated raw minced bee f after enrichment in modified tryptone soy broth (MTSB) incubated at 42 degrees C for only 4 hours. Compared to CHROMagar E. coli O157, sor bitol Mac-Conkey agar supplemented with cefixime and tellurite (CT-SMA C) was shown to be a better subculture medium.