C. Silversand et C. Haux, IMPROVED HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR THE SEPARATION AND QUANTIFICATION OF LIPID CLASSES - APPLICATION TO FISH LIPIDS, Journal of chromatography B. Biomedical sciences and applications, 703(1-2), 1997, pp. 7-14
Citations number
24
Journal title
Journal of chromatography B. Biomedical sciences and applications
An improved straight-phase HPLC method for the separation and quantifi
cation of lipid classes is described. Two binary gradient solvent syst
ems were used, one for polar and one for neutral lipids, and detection
was performed with a light-scattering detector. The developed HPLC me
thods were highly reproducible and allowed base-line separation of all
investigated polar lipid classes (phosphatidic acid, diphosphatidylgl
ycerol, phosphatidylethanolamine, phosphatidylcholine, sphingomyelin,
phosphatidylserine, phosphatidylinositol and lysophosphatidylcholine)
and neutral lipid classes (triacylglycerol, free fatty acid, diacylgly
cerol, cholesterol and monoacylglycerol) except of cholesterol ester a
nd wax ester. Application of the chromatographic systems demonstrated
that the methods are suitable for quantitative analysis of the major l
ipid classes present in lipid extracts from livers and eggs of Atlanti
c salmon (Salmo salar). (C) 1997 Elsevier Science B.V.