SIMULTANEOUS ANALYSIS OF LIGNOCAINE AND BUPIVACAINE ENANTIOMERS IN PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

Authors
ABRAHAM I FAWCETT JP KENNEDY J KUMAR A LEDGER R
Citation
I. Abraham et al., SIMULTANEOUS ANALYSIS OF LIGNOCAINE AND BUPIVACAINE ENANTIOMERS IN PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY, Journal of chromatography B. Biomedical sciences and applications, 703(1-2), 1997, pp. 203-208
Citations number
17
Journal title
Journal of chromatography B. Biomedical sciences and applications
ISSN journal
13872273 → ACNP
Volume
703
Issue
1-2
Year of publication
1997
Pages
203 - 208
Database
ISI
SICI code
0378-4347(1997)703:1-2<203:SAOLAB>2.0.ZU;2-7
Abstract
A sensitive analytical procedure is described for the simultaneous det ermination of lignocaine and the enantiomers of bupivacaine in biologi cal fluids using diazepam as an internal standard. After solvent extra ction into hexane, the local anaesthetics were separated using an alph a(1)-acid glycoprotein (AGP) column and detected at 214 nm. Calibratio n curves were linear (r(2)>0.99) in the concentration range of 5 to 50 0 ng/ml for the enantiomers of bupivacaine and 12.5 to 1000 ng/ml for lignocaine. The corresponding limits of detection were 4 ng/ml and 10 ng/ml, respectively. The method was applied to the analysis of plasma from a healthy woman undergoing tubal ligation. (C) 1997 Elsevier Scie nce B.V.