Citation
A. Fioravanti et al., HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF OROTIC-ACID AS ITS METHYL DERIVATIVE IN HUMAN URINE, Journal of chromatography B. Biomedical sciences and applications, 703(1-2), 1997, pp. 263-266
Citations number
8
Journal title
Journal of chromatography B. Biomedical sciences and applications
ISSN journal
13872273
→ ACNP
Volume
703
Issue
1-2
Year of publication
1997
Pages
263 - 266
Database
ISI
SICI code
0378-4347(1997)703:1-2<263:HLDOOA>2.0.ZU;2-W
Abstract
Described is a method for the determination of erotic acid as its meth
yl ester in human urine. The method involves the use of solid-phase ex
traction to isolate pyrimidines from urine and derivatization with met
hanol and sulfuric acid, followed by isocratic high-performance liquid
chromatography on a reversed-phase C-18 column with UV absorbance det
ection. The assay is shown to be sufficiently sensitive for use in cli
nical investigations where elevated erotic acid excretion is suspected
. (C) 1997 Elsevier Science B.V.