PLATELETAPHERESIS - COMPARISON OF PROCESSING TIMES, PLATELET YIELDS, AND WHITE BLOOD-CELL CONTENT WITH SEVERAL COMMONLY USED SYSTEMS

In this study we compare processing times and platelet yields of eight systems: five double-needle (DN)-Fenwal CS3000 Plus with interface of fset (IO) of 6 (Fen 6) and IO of 10 (Fen 10), a prototype Fenwal Amicu s (AM-DN), Spectra version 4 (Spec 4-DN) and version 5 with leukoreduc tion system (Spec 5-LRS); and three single-needle (SN) systems-Haemone tics MCS Plus (MCS+), prototype Amicus (AM-SN), and Spectra version 4 (Spec 4-SN). Twenty-five procedures from each of the systems (except A M-SN, N = 13) were compared; each system had comparable preprocedure p latelet counts and similar endpoints. Five systems-MCS+, Fen 6, Fen 10 with leukoreduction filter (MCS + LRF), (Fen 6 LRF), (Fen 10 LRF), as well as AM-DN, and Spec 5-LRS-were compared for WBC content. P-select in expression was compared in the MCS+; Fen-10 with A-35 (Fen 10-A35) and PLT-30 (Fen 10-PLT30) collection chambers; Spec 4-DN; and platelet -rich plasma. We found AM-DN (63 +/- 16 min) had a significantly lower average processing time than all other systems except AM-SN. Spectra 4-DN (5.6 +/- 1.7 x 10(11) Pit) produced significantly more platelets and had a significantly higher incidence of products greater than or e qual to 6.0 x 10(11) (56%) and higher incidence of products greater th an or equal to 6.6 x 10(11) Plt (32%) than all other systems. AM-DN (0 .073 +/- 0.017 x 10(11) Plt/min) had a significantly higher collection rate than all other systems except AM-SN. The WBC content comparison indicated MCS+ with a filter; and AM-DN and Spectra 5-LRS without filt ers were capable of consistently (99.32-100%) producing products with <5 x 10(6) WBC, but were less consistent (86.36-99.26%) at <1 x 10(6) WBC. MCS+ and Fen 10-PLT30 had significantly (p < .05) less p-selectin expression than all other systems but not between each other. From th e data in this study, if leukoreduced platelets were needed in the sho rtest processing time, the first and second choices would be Amicus-DN and Spectra 5-LRS, respectively. (C) 1997 Wiley-Liss, Inc.