CRITICAL-ASSESSMENT OF METHODS USED TO MEASURE PROTEIN-SYNTHESIS IN HUMAN-SUBJECTS

The loss of body protein that frequently accompanies illness occurs th rough changes in protein synthesis and degradation. In human tissues, rates of protein synthesis can be assessed with stable isotopic tracer techniques and mass spectrometry. The basic principles of these metho ds are explained, and the advantages and drawbacks of the two main app roaches, the constant infusion method and the flooding method, are des cribed. Examples are given of the use of these methods to investigate protein synthesis and surgical trauma, pathologies of the gastrointest inal tract and the response of tumor growth to amino acid supplements.