Citation
M. Hay et P. Mormede, IMPROVED DETERMINATION OF URINARY CORTISOL AND CORTISONE, OR CORTICOSTERONE AND 11-DEHYDROCORTICOSTERONE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH ULTRAVIOLET ABSORBENCY DETECTION, Journal of chromatography B. Biomedical sciences and applications, 702(1-2), 1997, pp. 33-39
Citations number
37
Journal title
Journal of chromatography B. Biomedical sciences and applications
ISSN journal
13872273
→ ACNP
Volume
702
Issue
1-2
Year of publication
1997
Pages
33 - 39
Database
ISI
SICI code
0378-4347(1997)702:1-2<33:IDOUCA>2.0.ZU;2-2
Abstract
A sensitive assay was developed for the determination of low levels of
free (unconjugated) glucocorticoids in human, swine (cortisol and cor
tisone) and rat urine (corticosterone and 11-dehydrocorticosterone), u
sing solid-phase extraction and HPLC with UV absorbance detection (254
nm). Precise quantitation is allowed by the use of internal standards
(dexamethasone for swine urine and Reichstein's substance S for rat u
rine). This simple method allows the use of small urine samples (less
than 2 ml), and is suitable for a wide range of applications in human
and animal clinical and physiological studies. (C) 1997 Elsevier Scien
ce B.V.