DETERMINATION OF CIS-THYMINE GLYCOL IN DNA BY GAS-CHROMATOGRAPHY MASS-SPECTROMETRY WITH SELECTED-ION RECORDING AND MULTIPLE REACTION MONITORING

Authors
FAROOQ S BAILEY E FARMER PB JUKES R LAMB JH HERNANDEZ H SRAM R TOPINKA J
Citation
S. Farooq et al., DETERMINATION OF CIS-THYMINE GLYCOL IN DNA BY GAS-CHROMATOGRAPHY MASS-SPECTROMETRY WITH SELECTED-ION RECORDING AND MULTIPLE REACTION MONITORING, Journal of chromatography B. Biomedical sciences and applications, 702(1-2), 1997, pp. 49-60
Citations number
55
Journal title
Journal of chromatography B. Biomedical sciences and applications
ISSN journal
13872273 → ACNP
Volume
702
Issue
1-2
Year of publication
1997
Pages
49 - 60
Database
ISI
SICI code
0378-4347(1997)702:1-2<49:DOCGID>2.0.ZU;2-4
Abstract
A novel method for the determination of cis-thymine glycol in DNA has been developed, using gas chromatography-mass spectrometry with select ed ion recording or multiple reaction monitoring. The procedure involv es acidic hydrolysis of DNA in the presence of the internal standard c is-[H-2(3)]thymine glycol, followed by derivatisation with hyl-N-(tert -butyldimethylsilyl)trifluoroacetamide. The method was validated on DN A that had been oxidatively modified in vitro by radiation treatment, and was then applied to determine cis-thymine glycol in human placenta l DNA. Background levels of 5.45+/-2.98 ng cis-thymine glycol/mg DNA w ere observed in the human samples. (C) 1997 Elsevier Science B.V.