MICROASSAY FOR DETERMINATION OF ITRACONAZOLE AND HYDROXYITRACONAZOLE IN PLASMA AND TISSUE BIOPSIES

A simple, rapid and sensitive method for the extraction and HPLC analy sis of itraconazole and hydroxyitraconazole in tissue and plasma or se rum is described. Tissue (5-100 mg) and plasma (0.1 ml) underwent a si mple extraction into methanol. Chromatography was performed on a Novap ak C-18 column using a mobile phase of water-acetonitrile-diethylamine (42:58:0.05, v/v), pH 2.45, with a flow-rate of 1.5 ml/min. Fluoresce nce was measured at excitation 260 nm and emission 365 nm. The procedu re produced a Linear curve for the concentration range 10-1000 ng/ml. The development of the assay produced accurate, rapid repeatable resul ts for both tissue and plasma or serum. (C) 1997 Elsevier Science B.V.