Jd. Alche et Mi. Rodriguezgarcia, FLUOROCHROMES FOR DETECTION OF CALLOSE IN MEIOCYTES OF OLIVE (OLEA-EUROPAEA L.), Biotechnic & histochemistry, 72(6), 1997, pp. 285-290
The callosic wall which covers microsporocyte mother cells during meio
tic division has been studied using different fluorochromes as alterna
tives to the widely used aniline blue. We have confirmed that both acr
idine orange and 4', 6' diamidino-2-phenylindole (DAPI) produce a fluo
rescent response to callose which is comparable in specificity and int
ensity to that of aniline blue; therefore, they can be used to study c
allose wall formation. Staining properties of these fluorochromes, as
well of those of curcumin and sirofluor, reported earlier as fluoresce
nt stains for callose, are discussed. We also discuss the efficacy of
the combined use of sirofluor and DAPI to study particular aspects of
the deposition of callose.