DEVELOPMENT OF LINSEED FOR INDUSTRIAL PURPOSES VIA PEDIGREE-SELECTIONAND HAPLOID-TECHNIQUE

The present experiments aimed to improve breeding methodology by using different routes for the development of pure lines. Induction media w ith different nitrogen sources were tested to improve the efficiency o f microspore culture technique in linseed (Linum usitatissimum L.). In comparison to the anther culture method applied simultaneously the ca llus production rate remained lower. But shoot differentiation from ca lli originating from microspore culture was more efficient. By compari ng doubled haploid (DH) lines and pedigree lines it could be demonstra ted that the progenies of three crosses which had been developed by pe digree selection showed a higher performance than simultaneously devel oped DH lines. In three other crosses pedigree lines (F-5 generation) and DH lines were compared under field conditions. Concerning seed yie ld, disease resistance and 1000-grain-weight, the pedigree lines exhib ited a superior performance as compared to the DH lines. In the cross 'Kubanskii x DH 57/1' DH lines could be identified that achieved highe r C18:3 contents than their parents. (C) 1998 Elsevier Science B.V.