Vh. Conzonno et Af. Cirelli, ISOLATION AND PURIFICATION OF FULVIC-ACIDS FROM A PAMPASIC POND (ARGENTINA), Fresenius environmental bulletin, 7(1-2), 1998, pp. 91-95
A method for the isolation of humic substances from El Burro Pond (Arg
entina) is described. Only fulvic acids are present due to the chemica
l composition of the water. The procedure is based on the adsorption-d
esorption through a macroporous resin (Amberlite XAD-2), neutralizatio
n of the eluate, desalting (Sephadex G-15), ion exchange (Amberlite IR
A-120, H-form) and lyophilization. The low ash content of the dried ma
terial indicates the effectiveness of the procedure.