COMPARISON OF METHODS FOR DETERMINATION OF VITAMIN-B-12 IN MICROBIAL MATERIAL

Authors
QUESADACHANTO A SCHMIDMEYER AC SCHROEDER AG FUCHTER A CARVALHOJONAS MF KOEHNTOPP PI JONAS R
Citation
A. Quesadachanto et al., COMPARISON OF METHODS FOR DETERMINATION OF VITAMIN-B-12 IN MICROBIAL MATERIAL, Biotechnology techniques, 12(1), 1998, pp. 75-77
Citations number
14
Categorie Soggetti
Biothechnology & Applied Migrobiology","Biochemical Research Methods
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
12
Issue
1
Year of publication
1998
Pages
75 - 77
Database
ISI
SICI code
0951-208X(1998)12:1<75:COMFDO>2.0.ZU;2-H
Abstract
Three different methods for the measurement of vitamin B-12 were compa red: two spectrophotometric methods and an HPLC one. When the pure vit amin was used, the results obtained using all three methods were simil ar, but when samples from microbial material were used, the results we re different. The HPLC method could distinguish the true vitamin B-12 from the different vitamin B-12 analogues whereas the spectrophotometr ic methods could not.