SUPPLY OF THE ARGU GENE-PRODUCT ALLOWS HIGH-LEVEL EXPRESSION OF RECOMBINANT HUMAN INTERFERON-ALPHA-2A IN ESCHERICHIA-COLI

Authors
JEONG W SHIN HC
Citation
W. Jeong et Hc. Shin, SUPPLY OF THE ARGU GENE-PRODUCT ALLOWS HIGH-LEVEL EXPRESSION OF RECOMBINANT HUMAN INTERFERON-ALPHA-2A IN ESCHERICHIA-COLI, Biotechnology letters, 20(1), 1998, pp. 19-22
Citations number
20
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology letters → ACNP
ISSN journal
01415492
Volume
20
Issue
1
Year of publication
1998
Pages
19 - 22
Database
ISI
SICI code
0141-5492(1998)20:1<19:SOTAGA>2.0.ZU;2-7
Abstract
Escherichia coli BL21 (DE3) co-transformed with the gene of human inte rferon-alpha 2a (IFN-alpha 2a) and the argU gene that codes for minor tRNA(Arg(AGG/AGA)) produced IFN-alpha 2a in yield of more than 25% of the total cellular proteins. IFN-alpha 2a was purified from insoluble inclusion bodies as much as 10 mg per liter of LB culture and the meth ionine residue at the N-terminus was found to be completely processed.