BIOSYNTHESIS AND ANALYSIS OF 3Z-NONENAL

Nonenal synthesis from 9(S)-hydroperoxy-linoleic acid by hydroperoxide -lyase-catalysed cleavage was followed by a precise headspace analysis with GC-MS. Enzyme activity was extracted from plant leaves and fruit s. 3Z-nonenal was the main product and a small amount of 2E-isomer was detected since the reaction-analysis procedure used avoided supplemen tary volatile fraction transformation. The substrate transformation an d volatile purge were carried out successively and without delay or an y contact with air.