CONDITIONED MEDIUM FROM HETEROGENEOUS PLANTS (ROSE AND GRAPE) ON CELL-GROWTH AND ANTHOCYANIN SYNTHESIS OF FRAGARIA-ANANASSA

Rose and grape cells were cultured under 800 lux for 1 week, and these broths were used as conditioned medium for stimulate anthocyanin synt hesis of strawberry suspension cells. Anthocyanin synthesis in strawbe rry cells was enhanced to produce 1000 mu g/g cells after 2 days of cu lturing in the conditioned medium prepared from white grape callus whi ch does not produce anthocyanin, and the enhancement was significantly (p < 0.05) greatest and it was 5 fold greater than that of the contro l medium. Stimulation by conditioned media also showed a concentration -dependent response for anthocyanin synthesis, although there was only a slight difference (4.0-4.8 g) in cell growth among the heterogeneou s conditioned media after 2 days of culturing. However after 5 days, c ell growth using 50% of conditioned media from red grape callus was 14 .6 g cell weight and 15 g in the control.